What is difference between C18 and Phenyl column?
What is difference between C18 and Phenyl column?
C18 has 18 carbon atoms while C8 has only 8 carbon atoms. C18 has a longer carbon chain, but C8 has a shorter one. C18 has higher retention while C8 has shorter retention. C18 has higher hydrophobicity, but C8 has a lower hydrophobicity….Follow Pharmaguideline.
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What is SB phenyl column?
Agilent ZORBAX SB-Phenyl is made by chemically bonding a sterically protected phenyl stationary phase to special ZORBAX silica, designed to reduce or eliminate strong adsorption of basic compounds. ZORBAX SB-Phenyl is especially suited for MS applications.
What is a SB C18 column?
General Description. Zorbax SB-C18 is a unique microparticulate C18 pack- ing used for reversed-phase high performance liquid chromatography. The StableBond packing is made by chemically bonding a sterically-protected C18 stationary phase to a specially prepared, high purity Zorbax po- rous silica microsphere.
Why phenyl columns are used?
Phenyl-type columns provide strong pi-pi interaction and offer selectivity complementary to other reversed-phase columns (e.g., C18). Recently, a biphenyl phase was commercialized that exhibited both hydrophobic retention and pi-pi interaction compared to other commercially available phenyl columns.
When would you use a phenyl-hexyl column?
Phenyl-Hexyl Phase Information Luna Phenyl-Hexyl columns offer increased retention for polar, aromatic compounds and can be used for applications that require reversals in elution order. Offers great stability as well as alternative selectivity.
What is column in HPLC?
Columns are the main component in HPLC because the column is responsible for the separation of the sample components. The sample passes through the column with the mobile phase and separates in its components when it comes out from the column. The material filled in the HPLC columns is known as a stationary phase.
What are the columns in zorbax eclipse XDB?
Agilent ZORBAX Eclipse XDB columns – C18, C8, Phenyl and CN – provide four bonded phase choices for method development optimization. These columns provide good peak shape over a wide pH range (2–9) for additional method development flexibility with one family of columns.
What does Agilent zorbax eclipse XDB-phenyl do?
Agilent ZORBAX Eclipse XDB-Phenyl. Description Agilent ZORBAX Eclipse XDB-Phenyl provides different selectivity from XDB-C18 and XDB-C8 and is an alternative to those phases for the separation of acidic, basic and other highly polar compounds.
Which is the best zorbax column to use?
ZORBAX Eclipse Plus columns are the recommended starting point for method development and benefit from the ultimate performance from fully porous silica-based columns
How does the Agilent zorbax extend-c18 column work?
The Agilent ZORBAX Extend-C18 column uses a novel bidentate C18-C18 bonding technology to make it possible to develop high-resolution separations at high pH with a silica-based column. At high pH, non-charged basic compounds will not interact with the underlying silica.