What is the reaction that the enzyme alkaline phosphatase catalyzes?
Alkaline phosphatase (ALP; E.C. 3. I. 3.1.) is an ubiquitous membrane-bound glycoprotein that catalyzes the hydrolysis of phosphate monoesters at basic pH values.
What does alkaline phosphatase do to DNA?
Alkaline phosphatase is the enzyme that catalyzes a dephosphorylation of DNA, RNA, ribo-, and deoxyribonucleoside triphosphates. It can also remove phosphates from nucleotides and proteins. They are most active at a basic pH.
What is the role of alkaline phosphatase enzyme?
Alkaline phosphatase (ALP) is an enzyme in a person’s blood that helps break down proteins. The body uses ALP for a wide range of processes, and it plays a particularly important role in liver function and bone development.
How does phosphate inhibit alkaline phosphatase?
Inorganic phosphate (Pi) and p-nitrophenol, the products of the hydrolysis of p-nitrophenyl phosphate by alkaline phosphatase, both competitively inhibit the enzyme. Lineweaver-Burk plots that show the same Vmax but increased Km in the presence of the inhibitor are characteristic of competitive inhibition.
What kind of enzyme is alkaline phosphatase?
Alkaline phosphatase is homodimeric enzyme, meaning it is formed with two molecules. Three metal ions, two Zn and one Mg, are contained in the catalytic sites, and both types are crucial for enzymatic activity to occur.
What is the purpose of DNA phosphatase?
This enzyme plays an important role in the growth and development of bones and teeth. It is also active in many other tissues, particularly in the liver and kidneys. This enzyme acts as a phosphatase, which means that it removes clusters of oxygen and phosphorus atoms (phosphate groups) from other molecules.
What does it mean if your alkaline phosphatase is high?
What do the results mean? High alkaline phosphatase levels may mean there is damage to your liver or that you have a type of bone disorder. Liver damage creates a different type of ALP than bone disorders do.
Why is phosphate an inhibitor?
Enzymes and Enzyme Regulation In the alkaline phosphatase reaction, in which hydrolysis of a wide variety of organic monophosphate esters into the corresponding alcohols (or phenols) and inorganic phosphates occurs, the inorganic phosphate acts as a competitive inhibitor.
What type of inhibitor is phosphate for acid phosphatase?
Fluoride and phosphate are well-known inhibitors of acid phosphatase. Also in our study, they acted as inhibitors for enzyme activity. These effectors exhibited mixed and competitive type of inhibition on acid phosphatase activity, respectively (Figures 6 and 7).
When does pNPP react with alkaline phosphatase?
When alkaline phosphatase and PNPP are reacted, a yellow water-soluble reaction product is formed. This reaction product absorbs light at 405nm. The substrate is offered in several formats. For Research Use Only. Not for use in diagnostic procedures.
What is the pNPP reaction product in Elisa?
PNPP ( p -Nitrophenyl Phosphate, Disodium Salt) is a widely used substrate for detecting alkaline phosphatase in ELISA applications. When alkaline phosphatase and PNPP are reacted, a yellow water-soluble reaction product is formed. This reaction product absorbs light at 405nm.
How to stop the pNPP reaction in NaOH?
Start the reaction by adding 0.24 ml of an enzyme solution. ２）After the reaction times of 1, 10, and 20 min, respectively, stop the reaction by adding 0.6 ml of 0.5 M NaOH. NaOH stops the reaction and converts the pNP produced into a yellow-colored (A400-absorbing) form.
Which is pNPP reaction product absorbs light?
When alkaline phosphatase and PNPP are reacted, a yellow water-soluble reaction product is formed. This reaction product absorbs light at 405nm. The substrate is offered in several formats.