What is the purpose of 1 kb DNA ladder?
Invitrogen 1 Kb Plus DNA Ladder is designed for sizing and approximate quantification of double-stranded DNA in the range of 100 bp to 15,000 bp. 1 Kb Plus DNA Ladder consists of 18 individual chromatography-purified DNA fragments and has a reference band at 1,500 bp for easy orientation.
What does KB stand for in gel electrophoresis?
A measure of DNA chain length; 1000 nucleotide residues.
How much is 1kb of DNA ladder?
We recommend loading 10 μl (0.5 μg) of Quick-Load 1 kb DNA Ladder per gel lane. In general, it is recommended to load 1µl per mm of gel lane.
What is a DNA size standard?
A DNA marker (also known as a size standard or a DNA ladder) is loaded into the first well of the gel. The fragments in the marker are of a known length so can be used to help approximate the size of the fragments in the samples.
When to use generuler 1 kb DNA ladder?
In April 2018 this product was upgraded to include TriTrack DNA Loading Dye for better visualization. Thermo Scientific GeneRuler 1 kb DNA Ladder is recommended for sizing and approximate quantification of a double-stranded DNA in the range of 250 bp to 10,000 bp on agarose gels.
How many DNA fragments are in 1 KB plus generuler?
Description Thermo Scientific GeneRuler 1 kb Plus DNA Ladder is recommended for sizing and approximate quantification of a double-stranded DNA in the range of 75 bp to 20,000 bp on agarose gels. The DNA ladder consists of 15 DNA fragments and is provided with 6X TriTrack DNA Loading Dye. Highlights of 1 kb Plus DNA Ladder
How many DNA fragments are in a DNA ladder?
The DNA ladder consists of 14 DNA fragments and is provided with 6X TriTrack DNA Loading Dye. This double-stranded DNA ladder is compatible with 1% standard and precast agarose slab gels and can be visualized after ethidium bromide or SYBR Safe staining. A uniform intensity of bands and bright reference bands allow easy sizing and orientation.
What kind of DNA is in the Invitrogen DNA ladder?
Invitrogen DNA ladders are composed of double-stranded DNA fragments only. Are there any suggestions to improve the detection of the bands of my DNA molecular weight ladder in an agarose gel? (1) An insufficient quantity or concentration of DNA loaded on the gel. You can increase the amount of DNA.